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Research Associate / Research Institute Scientist I


RTW Charitable Foundation

New York, NY

Posted August 8

Job Description


The RTWCF is proud to support pioneers who are breaking new ground in the fight against diseases that may not represent compelling commercial opportunities but do result in significant human suffering. RTWCF’s mission is to power rare disease research, medical innovation, and humanitarian collaborations to improve the health of underserved communities. The goal of the RTWCF Research Institute (RI) is to conduct transformative basic and translational research to generate key experimental data on under-resourced rare diseases to advance potential therapeutic paths forward for these patients. The Foundation was created at the intersection of scientific advancements and humanitarian efforts for all people to live healthier, longer lives. The RTWCF aims to provide direct support to patients and their families.

We are seeking a Scientist/Senior Research Associate to support the production, engineering, and characterization of AAV vectorized gene therapies for nonclinical development efforts. The successful candidate will have experience in molecular biology techniques (DNA manipulation and purification) and mammalian cell culture techniques, with preference given to individuals with experience in AAV vector production/purification and/or other relevant experience with nucleic acid-based medicines (other viral vectors, ASO, siRNA, mRNA, etc.). This is an exciting opportunity to join the expansion of a non-profit research institute and apply advancements in genetic-based medicines toward patients with rare disease.

 

Primary Responsibilities include:

  • Report to and work directly with the Research Institute Director
  • Tissue culture production, processing, and purification of viral vectors for preclinical efforts
  • Molecular biology assays (cloning: Gibson assembly, Gateway, TA, PCR-overhang & RE-ligation; PCR: end point, RT-PCR, qPCR, dPCR, ddPCR; gel electrophoresis; Western Blot; ELISAs; etc.)
  • Mammalian cell culture & aseptic techniques, maintenance, isolation/differentiation protocols, and common manipulations (transfection, transduction, electroporation, etc.)

 

Requirements:

  • MS. with >2-3yrs of relevant industry/academic experience (or a B.S. with >5yrs)
  • Produce, purify, quantify, and characterize viral vectors (preference given to AAVs)
  • Familiar with gradient ultra-centrifugation & affinity chromatography purification techniques
  • Nucleic acid manipulation techniques, plasmid/construct generation, and DNA sequence analysis
  • Implement and develop assays to characterize and assess viral vectors or nucleic acid-based drugs
  • Evaluate in vitro virus transduction and transgene expression
  • Familiar with bioassay development, screening/HTS assay design & optimization
  • Perform basic biochemical/immunological assays (protein/DNA gels, ELISA, flow cytometry)
  • Analysis & presentation of data in a team-based environment
  • Proper documentation of experiments, maintenance of accurate, up-to-date lab records
  • Maintenance of lab supplies/equipment, maintain a clean and organized work environment
  • Exceptional attention to detail and organizational skill, independently plan & execute work
  • Stay abreast of the most current literature related to ongoing projects
  • Familiar with software and programs: Microsoft Office, Graphpad Prism, DNA analysis software, and online resources (NCBI sites, UCSC Genome Browser, OMIM, UniProt, etc)

 

Job Type

  • Full-time
  • On-Site

Salary

  • Commensurate with experience


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